rna-seq data protein atlas version 23 Search Results


90
Human Protein Atlas human protein atlas rna-seq dataset
Human Protein Atlas Rna Seq Dataset, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas rna-seq data
Rna Seq Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas tcga rna-seq data
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
Tcga Rna Seq Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas tpm-normalized rna-seq expression data of gapdhs
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
Tpm Normalized Rna Seq Expression Data Of Gapdhs, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas whole transcriptome data [rna-seq]
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
Whole Transcriptome Data [Rna Seq], supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas rna-seq based gene expression data
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
Rna Seq Based Gene Expression Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas human protein atlas rna sequencing data
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
Human Protein Atlas Rna Sequencing Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas gtex nrsf and crh rna-seq data
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
Gtex Nrsf And Crh Rna Seq Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas high-level transcripts per kilobase per million mapped reads (tpm) rna-seq data
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
High Level Transcripts Per Kilobase Per Million Mapped Reads (Tpm) Rna Seq Data, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas deep sequencing of rna
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
Deep Sequencing Of Rna, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas rna-seq data schmiedel dataset
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
Rna Seq Data Schmiedel Dataset, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas rna-seq data schmeidel dataset
( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from <t>TCGA).</t> The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).
Rna Seq Data Schmeidel Dataset, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from TCGA). The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).

Journal: Science Advances

Article Title: Tumor-specific cytolytic CD4 T cells mediate immunity against human cancer

doi: 10.1126/sciadv.abe3348

Figure Lengend Snippet: ( A ) tSNE 2D plot of scRNA-seq data representing the four CD4 + T cell clusters discriminating the cytolytic (blue), memory (green), cycling (yellow), and regulatory (gray) subsets and their expression of selected genes (SLAMF7, PDCD1, PRF1, GZMA, GZMB, GZMH, GZMK, GZMM, and granulysin). ( B ) Quantitative RT-PCR analysis of the indicated transcripts in ex vivo–sorted NY-ESO-1 87–99 /DR7 CD4 T cells from either PB or TILs/TILNs. ( C ) Representative examples and cumulative analysis of direct ex vivo NY-ESO-1 87–99 /DR7 CD4 T cell multimer staining combined with the evaluation of SLAMF7 and PD-1 expression of either PB or TILs/TILNs from patients with melanoma. ( D ) qRT-PCR analysis of the indicated transcripts in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell clones discriminated as Th or Th-CTX. ( E ) Representative examples and cumulative analysis of SLAMF7 and PD-1 expression in PB-derived NY-ESO-1 87–99 /DR7 CD4 T cell Th and-Th-CTX clones. ( F ) Standard 4-hour 51 Chromium release assay, conducted with target tumor cell lines transduced with CIITA pulsed with the specific peptide in the presence or absence of Nivolumab (10 μg/ml) (left) or agonistic anti-SLAMF7 antibody (5 μg/ml) (right) and coincubated with antigen-specific CD4 T cell clones. Results are expressed as percentage of specific lysis of the target cells at 30:1 E:T ratios. ( G ) Kaplan-Meier plot depicting the survival probability and 95% confidence interval over time (years) for melanoma patients with high (blue) and low (black) SLAMF7 expression (data originated from TCGA). The difference between the two survival curves was assessed using a log-rank test. Statistics were performed using two-way ANOVA with Sidak’s post hoc test (B to D), nonparametric Mann-Whitney test (E), and Wilcoxon test (F).

Article Snippet: The correlation between the SLAMF7 expression level and survival of patients with cancer was performed by using TCGA RNA-seq data available on the Human Protein Atlas website ( www.proteinatlas.org ).

Techniques: Expressing, Quantitative RT-PCR, Ex Vivo, Staining, Derivative Assay, Clone Assay, Release Assay, Transduction, Lysis, MANN-WHITNEY